Implementing high-throughput insect barcoding in microbiome studies: impact of non-destructive DNA extraction on microbiome reconstruction
V. Andriienko, M. Buczek, R. Meier, A. Amrita Srivathsan, P. Lukasik, M. R. Kolasa
This study evaluates the impact of non-destructive DNA extraction using HotSHOT on microbiome reconstruction in insect specimens, finding it suitable for community composition but less reliable for abundance estimates.
bioRxiv · Apr 30, 2024Microbiology