M. Gantz

Fluorogenic, sub-single-turnover monitoring of enzymatic reactions involving NAD(P)H provides a generalised platform for directed ultrahigh throughput evolution of biocatalysts in microdroplets

The paper develops a fluorogenic assay for NAD(P)H detection to enable high-throughput screening of biocatalysts in microdroplets, demonstrating improved sensitivity and applicability for glycosyl hydrolases and IREDs.

Selection of a Promiscuous Minimalist cAMP Phosphodiesterase from a Library of De Novo Designed Proteins

The paper screens a library of de novo designed proteins to identify a promiscuous cAMP phosphodiesterase, finding that truncation and structural dynamics enable catalytic activity.

Ultrahigh-throughput Absorbance Activated Droplet Sorting (UHT-AADS) for enzyme screening at kilohertz frequencies

This paper introduces UHT-AADS, a method that improves absorbance-based droplet sorting speed to match fluorescence sorting, enhancing enzyme screening throughput.

Sorting of droplets at kHz rates using absorbance activated acoustic sorting

This paper introduces an acoustic activated absorbance sorter that increases sorting speed to 1 kHz and improves signal quality by reducing scattering and refraction artefacts.