ThermoBRET: a ligand-engagement nanoscale thermostability assay applied to GPCRs
B. L. Hoare, D. N. Tippett, A. Kaur, S. A. Cullum, T. Miljus, E. J. Koers, C. R. Harwood, N. Dijon, N. D. Holliday, D. A. Sykes, D. B. Veprintsev
ThermoBRET quantifies GPCR thermostability using BRET without labelled ligands, revealing ligand effects on protein unfolding in non-purified samples.
bioRxiv · Aug 5, 2020Biochemistry