Waffle Method: A general and flexible approach for FIB-milling small and anisotropically oriented samples
K. Kelley, A. M. Raczkowski, O. Klykov, P. Jaroenlak, D. Bobe, M. Kopylov, E. T. Eng, G. Bhabha, C. S. Potter, B. Carragher, A. J. Noble
The paper introduces the Waffle Method to improve cryo-FIB/SEM workflows for preparing complex biological samples for cryo-ET, demonstrating its effectiveness with various samples and hardware.
bioRxiv · Oct 28, 2020Microbiology