B. Carragher

Early intermediates in bacterial RNA polymerase promoter melting visualized by time-resolved cryo-electron microscopy

This study uses time-resolved cryo-EM to visualize early intermediates in bacterial RNA polymerase promoter melting, revealing key steps in the formation of the transcription-competent open complex.

PDZD8-FKBP8 tethering complex at ER-mitochondria contact sites regulates mitochondrial complexity

This study identifies FKBP8 as the tethering partner of PDZD8 at ER-mitochondria contact sites, showing their interdependence in regulating mitochondrial morphology.

Extended regulation interface coupled to the allosteric network and disease mutations in the PP2A-B56δ holoenzyme

The paper investigates the structural and functional dynamics of the PP2A-B56δ holoenzyme, showing how mutations linked to intellectual disability affect its allosteric network and phosphorylation rates.

Fully Automated Multi-Grid Cryo-EM Screening using Smart Leginon

Smart Leginon automates cryo-EM grid screening, reducing operator time from 6 hours to <10 minutes and improving throughput.

Vitrocam: A simple low cost Vitrobot camera for assessing grid quality

The paper introduces Vitrocam, a low-cost camera to assess cryo-EM grid quality during sample preparation by imaging the Vitrobot process.

System Environmental Metrics Collector for EM facilities

The paper develops a system for monitoring environmental metrics in cryoEM facilities to optimize instrument uptime and data production.

Waffle Method: A general and flexible approach for FIB-milling small and anisotropically oriented samples

The paper introduces the Waffle Method to improve cryo-FIB/SEM workflows for preparing complex biological samples for cryo-ET, demonstrating its effectiveness with various samples and hardware.

Time-resolved cryoEM using Spotiton

The paper introduces a method for preparing cryoEM grids to study short-lived molecular states by rapidly dispensing sample drops and vitrifying them.

Cryo-EM Structures and Regulation of Arabinofuranosyltransferase AftD from Mycobacteria

The paper presents the cryo-EM structure of AftD from M. abscessus and shows its interaction with ACP, revealing potential regulatory mechanisms.

The impact of data reduction and lossy image formats on electron microscope images

This study examines how data compression and lossy image formats affect cryoEM reconstruction quality, finding that compressed data can still yield sub-2Å reconstructions.

High Resolution Single Particle Cryo-Electron Microscopy using Beam-Image Shift

This study evaluates the impact of beam-image shift on cryo-EM single-particle reconstruction quality by testing different beam tilt angles and analyzing FSC values and water density peaks.

Complete functional mapping of infection- and vaccine-elicited antibodies against the fusion peptide of HIV

The paper maps functional epitopes of antibodies targeting HIV Env fusion peptide by analyzing mutations affecting neutralization, revealing differences between vaccine- and infection-elicited antibodies.

Routine Determination of Ice Thickness for Cryo-EM Grids

The paper presents two methods to measure ice thickness in cryo-EM grids during data collection to improve sample preparation and data quality.

Routine Single Particle CryoEM Sample and Grid Characterization by Tomography

This study uses fiducial-less tomography to analyze cryoEM grids and finds that 90% of particles are adsorbed to air-water interfaces, impacting protein structure and single particle analysis.