cryo-EM

Bacterial major vault protein homologs form divergent vault structures

The paper identifies bacterial MVP homologs and reports cryoEM structures of vaults from Corallococcus praedator and a Deltaproteobacteria bacterium, revealing novel symmetries.

Inter-domain flexibility in F-Box proteins revealed by integrative modeling

The paper uses integrative modeling to reveal inter-domain flexibility in F-box proteins, showing varying conformational dynamics across different F-box proteins.

Cryo-EM structures of an Acinetobacter baumannii podovirus reveal a canonical capsid maturation

The paper reports the cryo-EM structures of an Acinetobacter baumannii podovirus, revealing its capsid maturation mechanism and providing a reference for phage engineering.

Stepwise assembly and mechanical stability of nsP3 Helical Scaffolds in Chikungunya Virus

This study investigates the stepwise assembly and mechanical stability of nsP3 helical scaffolds in Chikungunya Virus using HS-AFM and EM, revealing domain-specific contributions to scaffold formation and function.

Structural basis for inefficient G protein coupling by Frizzled receptors

The paper identifies detergent LMNG as stabilizing a weak FZD-G protein interface, revealing that FZDs inherently weakly couple to G proteins and preferentially signal through DVL.

The Molecular Interplay of Tau and Motor Proteins on Microtubules

This study investigates how tau protein regulates motor proteins on microtubules using single-molecule imaging, cryo-EM, and MD simulations, revealing distinct mechanisms for inhibiting kinesin-1 and dynein.

Decision benchmarking of cryo-EM re-refinement

The paper benchmarks cryo-EM re-refinement selection methods against no operation using a fixed cohort and scoring tools, finding increased disagreement with higher-resolution references.

The mechanism of cysteine sensing by LRRC58

This study identifies how LRRC58 senses cysteine through structural changes that regulate CDO1 binding and cysteine homeostasis.

Structural basis of ribosomal RNA repair by RtcB2

This study presents cryo-EM structures of RtcB2 bound to damaged E. coli 30S subunits, revealing how the enzyme recognizes and repairs ribosomal RNA.

Structural and evolutionary insights into FcαμR recognition of polymeric immunoglobulins

This study determines cryo-EM structures of FcR bound to IgM and IgA, revealing its binding mechanism and evolutionary insights into pIg receptor recognition.

Facilitating the identification of cryo-EM hit matter in drug discovery with Astex-Faro

Astex-Faro improves cryo-EM hit identification by detecting structural deviations and enhancing weak features across diverse datasets.

CryoID2: deep learning-enhanced identification of protein and RNA from cryoEM maps

CryoID2 uses deep learning and sequence analysis to identify proteins and RNA from cryoEM maps, achieving high accuracy in molecular identification.

Structural Basis for Inhibition of Human Anion Transporter SLC26A6/PAT-1

This study reports cryo-EM structures of human PAT-1 with inhibitors to elucidate their inhibition mechanisms and domain interactions.

Mapping AMPA and NMDA receptors at mouse brain hippocampal synapses by cryo-ET

The paper maps AMPA and NMDA receptors at mouse hippocampal synapses using cryo-ET, revealing their distribution and relationship to glutamate release sites.

Comparative cryo-EM structures of CGMMV and ToMMV reveal divergent coat protein packing and electrostatics indicating Tobamovirus host specificity

Oligodendrocytes may provide the extra density of α-synuclein filaments from multiple system atrophy

The paper investigates how oligodendrocytes contribute to the formation of α-synuclein filaments in multiple system atrophy by comparing seeded aggregation in different cell types.

Cryo-EM structures of Cereibacter sphaeroides ferrochelatase reveal an oligomeric state that occludes the substrate-binding site

This study determines the cryo-EM structures of Cereibacter sphaeroides ferrochelatase, revealing oligomeric states that occlude the substrate-binding site.

Particle picking and centering in cryo-electron micrographs via a vanishing radial kernel

The paper introduces a training-free method for particle picking and centering in cryo-EM by using a radial kernel convolution, achieving comparable reconstruction resolutions to reference-based methods.

End-to-end reconstruction for cryo electron ptychographic single-particle analysis

This paper introduces an end-to-end reconstruction algorithm for cryo-ptycho single-particle analysis, achieving better resolution than sequential methods on simulated data and some experimental datasets.

Structural basis for curvature generation and functional specialization in spirochete flagella

The paper determines the near-atomic structures of spirochete flagellar filaments, revealing how sheath proteins generate curvature and enable functional specialization.

Preserving Information and Integrity in Cryo-EM: A Fourier-Space Deposition Approach

The paper identifies limitations in current cryo-EM data deposition practices and proposes an alternative approach to better preserve information and support validation.

Molecular basis for ligand-gating of the human GluD1 receptor

This study determines the structure of human GluD1 and elucidates its ligand-gating mechanism using cryoEM and electrophysiology.

Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching

This paper improves cryo-EM reconstruction of small complexes using 2D template matching and high-resolution structures as priors, enhancing density of ligand-binding sites in a 43 kDa protein kinase.

CryoSHIELDs: tunable amphipathic peptide additives for cryo-EM

This paper introduces cryoSHIELDs, tunable peptides that improve cryo-EM sample preparation by modulating the air-water interface, enhancing particle distribution and complex integrity across multiple protein systems.

Cryo-EM structure of a methanogen nitrogenase-PII protein supercomplex

The paper reports a cryo-EM structure of a nitrogenase-PII supercomplex in methanogens, revealing a metabolite-gated assembly mechanism for nitrogenase inhibition.

Near-atomistic simulations reveal the molecular principles that control chromatin structure and phase separation

This study introduces OpenCGChromatin, a model that simulates chromatin structure and phase separation, revealing how linker-DNA length and histone modifications affect chromatin organization.

A conserved archaeal ribosome-associated factor linking bacterial hibernation and eukaryotic energy sensing

This study identifies AHA, a conserved archaeal ribosome-associated factor involved in hibernation and energy sensing, linking prokaryotic translational silencing to eukaryotic energy sensing.

Cryo-EM structure of the Arabidopsis thaliana V-type ATPase

The paper determines the Cryo-EM structure of the Arabidopsis thaliana V-type ATPase, revealing its catalytic cycle and organelle-specific interactions with TLDc proteins.

How the TREX-2 complex associates with the nuclear pore

The paper reports an extended structure of the human nuclear pore complex, revealing TREX-2 components as integral parts of the nuclear ring.

The archaic CUP pilus SMF-1 uses an antiparallel bundling mechanism to initiate biofilm formation

This study reveals the structure and bundling mechanism of SMF-1, a CUP pilus in Stenotrophomonas maltophilia, showing it forms antiparallel bundles that initiate biofilm formation.