Rapid Adeno-Associated Virus Genome Quantification with Amplification-Free CRISPR-Cas12a
Z. Hetzler, S. M. Marinakos, N. Lott, N. Mohammad, A. Lass-Napiorkowska, J. Kolbe, L. Turrentine, D. Fields, L. Overton, H. Marie, A. Hucknall, O. Rammo, H. George, Q. Wei
The paper develops a CRISPR-based assay to rapidly and accurately quantify AAV genome titers, enabling efficient manufacturing of rAAV vectors.
bioRxiv · Nov 14, 2023Bioengineering