Structure of the mini-RNA-guided endonuclease CRISPR-Cas{Phi}3
A. Carabias del Rey, A. Fuglsang, P. Temperini, T. Pape, N. Sofos, S. Stella, S. Erlendsson, G. Montoya
This paper determines the cryoEM structure of CRISPR-Cas{Phi}3, revealing its DNA targeting and cleavage mechanism, and shows how it enables unspecific ssDNA degradation.
bioRxiv · Jun 27, 2021Biochemistry