Efficient Generation of Humanized Cloned Cattle via Embryonic Stem Cell-Mediated Dual-Round Site-Specific Editing
Zhiqiang Feng, Jing Zhang, Shenao Tao, Peiru Lv, Xiaowei Zhang, S. H. Wang, Minglei Zhi, He Zhang, Chang Zeng, Xin Li, Zengyuan Zhao, Yanhua Li, Tianzhi Chen, Yadi Teng, Yuhan Yang, , Jianyong Han
This study develops a method using bovine epiblast stem cells for efficient gene editing and cloning of cattle, resulting in higher birth and survival rates compared to fibroblast-based cloning.
bioRxiv · Dec 24, 2025 · PreprintBioengineering