Massively parallel genomic perturbations with multi-target CRISPR reveal new insights on Cas9 activity and DNA damage responses at endogenous sites
R. S. Zou, A. Marin-Gonzalez, Y. Liu, H. B. Liu, L. Shen, R. Dveirin, J. X. J. Luo, R. Kalhor, T. Ha
This paper introduces multi-target gRNAs (mgRNAs) to enable high-throughput CRISPR screening, revealing insights into Cas9 dynamics, DNA damage responses, and chromatin changes at endogenous sites.
bioRxiv · Jan 18, 2022 · PreprintCell Biology