L. Shen

Follow-up availability and the robustness of immune transcriptomic recovery after myocardial infarction

The paper assesses the robustness of immune transcriptomic recovery after myocardial infarction by evaluating how missing data and assay availability affect inflammatory-response scores over time.

3D Brain Vascular Niche Model Captures Invasive Behavior and Gene Signatures of Glioblastoma

This paper develops a 3D brain vascular niche model to study GBM invasion and gene signatures, revealing glia-vascular contact influences transcriptional adaptation and poor prognosis.

Structures of PSI-FCPI from Thalassiosira pseudonana in high light provide convergent evolution and light-adaptive strategies in diatom FCPIs

The paper reports cryo-EM structures of PSI-FCPI complexes from Thalassiosira pseudonana under high light, revealing light-adaptive strategies and convergent evolution in diatom FCPIs.

Coxsackievirus group B3 regulates ASS1-mediated metabolic reprogramming and promotes macrophage inflammatory polarization in viral myocarditis

This study shows CVB3 infection upregulates ASS1 through citrulline consumption, leading to metabolic reprogramming and macrophage inflammatory polarization, contributing to viral myocarditis.

Automated Cytometric Gating with Human-Level Performance Using Bivariate Segmentation

This paper introduces UNITO, a deep learning framework for automated cytometric gating that matches human performance and provides interpretable results without requiring manual input.

An Engineered IGF2 Mutant for Lysosomal Targeting Chimeras Development and Membrane Proteins Degradation

This paper introduces eiLYTACs, an engineered IGF2-based technology that degrades membrane proteins and inhibits tumor growth by avoiding IGF1R activation.

Potential clinical value and influence of conductivity in conductive cardiac patches for reducing post-MI arrythmia risks

This study evaluates the clinical value of conductive cardiac patches by testing different conductivities and shows that higher conductivity reduces arrhythmia risks in post-MI hearts.

YAP1 status defines two intrinsic subtypes of LCNEC with distinct molecular features and therapeutic vulnerabilities

Inflammation-Induced Claudin-2 Upregulation Limits Pancreatitis Progression by Enhancing Tight Junction-Controlled Pancreatic Ductal Transport

This study shows CLDN2 upregulation during pancreatitis limits disease progression by enhancing tight junction-controlled fluid transport in pancreatic ductal epithelium.

Multi-omics map revealed PPARalpha activation protecting against myocardial ischemia-reperfusion injury by maintaining cardiac metabolic homeostasis

The paper identifies PPARalpha activation as protective against myocardial ischemia-reperfusion injury by maintaining cardiac metabolic homeostasis, using multi-omics data from rat hearts.

Molecular dynamics analyses of CLDN15 pore size and charge selectivity

The paper uses molecular dynamics simulations and cell culture experiments to study how the D55 residue in CLDN15 affects ion size and charge selectivity in tight junctions.

Lipid-anchored Proteasomes Control Membrane Protein Homeostasis

This study identifies N-myristoylation of Rpt2 as a mechanism for proteasome-membrane interaction, showing its role in membrane protein homeostasis and its impact on cellular processes and tumor growth.

Novel discovery of a lymphatic bridge connecting Schlemm's canal to limbal and conjunctival lymphatic pathway

The paper discovers a lymphatic bridge connecting Schlemm's canal to limbal and conjunctival lymphatic pathways using advanced imaging techniques in mice.

Targeted repression of DNA topoisomerase I by CRISPRi reveals a critical function for it in the Chlamydia trachomatis developmental cycle

This study uses CRISPRi to knock down topA in Chlamydia trachomatis, showing its critical role in developmental cycle and growth.

Multi-omics of the gut microbial ecosystem in the immunotherapy resistance in microsatellite instability-high gastrointestinal cancer patients

The paper uses multi-omics data to identify gut microbiome and metabolite markers of immunotherapy resistance in MSI-H/dMMR gastrointestinal cancer patients and develops a predictive model with 0.83 accuracy.

The Mechanism underlying B cell Developmental Dysfunction in Kawasaki Disease Based on Single-cell Transcriptomic Sequencing

This study uses single-cell transcriptomics to identify B cell developmental dysfunction in Kawasaki Disease, showing repression of immune processes and altered gene expression in HSPCs and B cells before treatment.

Integrated Cell Landscape and Dynamics in the Progression of Bone Repair

The paper maps cell landscape and dynamics in rodent bone repair using single-cell RNA sequencing and identifies functional roles of myeloid and lymphoid cells in osteogenesis and angiogenesis.

Porosity-permeability tensor relationship of closely and randomly packed fibrous biomaterials and biological tissues: Application to the brain white matter

This paper derives an anisotropic porosity-permeability tensor relationship for brain white matter and proposes a model to improve transport property design in fibrous biomaterials.

Comprehensive approach to study branched ubiquitinchains reveals roles for K48-K63 branches inVCP/p97-related processes

This paper develops a pipeline to study branched ubiquitin chains, identifies debranching enzymes, and reveals their roles in VCP/p97 processes and DNA damage responses.

Rice potassium transporter OsHAK18 mediates phloem K+ loading and redistribution

This study identifies OsHAK18 as a phloem-localized K+ transporter that mediates K+ loading and redistribution in rice, enhancing shoot K+ retention under low-K+ stress.

A SI3-sigma; arch stabilizes cyanobacteria transcription initiation complex

The paper reports cryo-EM structures of cyanobacterial transcription initiation complexes and identifies an SI3-{sigma} arch that stabilizes the RNAP-promoter DNA open complex, essential for transcription initiation.

Engineered nickel bioaccumulation in Escherichia coli by NikABCDE transporter and metallothionein overexpression

This study engineered Escherichia coli to enhance nickel bioaccumulation using overexpressed NikABCDE transporter and metallothionein, achieving 7-fold improvement but with reduced cell viability.

Cardiac patch treatment alleviates ischemic cardiomyopathy correlated with reverting Piezo1/2 expression by unloading left ventricular myocardium

This study shows cardiac patches reduce ischemic cardiomyopathy by lowering mechanical load and reverting Piezo1/2 expression in the left ventricle.

Spatial patterning and immunosuppression of glioblastoma immune contexture in hypoxic niches

This study identifies hypoxia-driven immunosuppression in glioblastoma by tracking TAM dynamics and reveals niche factors that promote immunotolerant states and poor prognosis.

Massively parallel genomic perturbations with multi-target CRISPR reveal new insights on Cas9 activity and DNA damage responses at endogenous sites

This paper introduces multi-target gRNAs (mgRNAs) to enable high-throughput CRISPR screening, revealing insights into Cas9 dynamics, DNA damage responses, and chromatin changes at endogenous sites.

OrganoID: a versatile deep learning platform for tracking and analysis of single-organoid dynamics

OrganoID automatically tracks and analyzes single-organoid dynamics in microscopy images, showing high accuracy in counting, sizing, and morphology analysis across multiple organoid types.

Molecular mechanism of claudin-15 strand flexibility

This study uses molecular dynamics simulations to investigate the flexibility of claudin-15 strands and identifies interfacial interaction networks as the source of their lateral flexibility.

Fungal auxin is a quorum-based modulator of blast disease severity

This study identifies fungal auxin as a quorum-sensing signal modulating rice blast disease severity through IAA biosynthesis and secretion.

OsMADS58 stabilizes gene regulatory circuits during rice stamen development

This study identifies OsMADS58's role in stabilizing gene regulatory circuits during rice stamen development using CRISPR knockout and transcriptomic analysis.

Distinct roles of glutamine metabolism in benign and malignant cartilage tumors with IDH mutations

This study shows that glutamine metabolism has different roles in benign and malignant cartilage tumors with IDH mutations, suggesting GLS inhibition as a potential therapy for chondrosarcoma.