A Vaccinia-based system for directed evolution of GPCRs in mammalian cells
C. Klenk, M. Scrivens, A. Niederer, S. Shi, L. Mueller, E. Gersz, M. Zauderer, E. Smith, R. Strohner, A. Plückthun
This paper develops a Vaccinia-based system to evolve GPCRs in mammalian cells, improving stability and expression, and demonstrating functional receptor properties.
bioRxiv · Oct 17, 2022Biochemistry