Per-pixel unmixing of spectrally overlapping fluorophores using intra-exposure excitation modulation
H. Valenta, F. Bierbuesse, R. Vitale, C. Ruckebusch, W. Vandenberg, P. Dedecker
The paper introduces exNEEMO, a method to quantify spectrally overlapping fluorophores using intra-exposure excitation modulation, enabling accurate per-pixel unmixing.
bioRxiv · Apr 29, 2023Biophysics