P. Dedecker

Novel Genetically Encoded Probes for Functional Imaging of Cell Signaling by Polarization Microscopy

This paper introduces FLIPs, a new class of genetically encoded biosensors that enable real-time single-cell imaging of cell signaling without modifying target proteins.

Per-pixel unmixing of spectrally overlapping fluorophores using intra-exposure excitation modulation

The paper introduces exNEEMO, a method to quantify spectrally overlapping fluorophores using intra-exposure excitation modulation, enabling accurate per-pixel unmixing.

The TriScan: fast and sensitive 3D confocal fluorescence imaging using a simple optical design

The paper introduces a fast and sensitive 3D confocal fluorescence microscope called TriScan, combining speed and resolution for various applications.

Fast and artifact-free excitation multiplexing using synchronized image scanning

The paper introduces a device called Resonator for fast, artifact-free fluorescence imaging with multiple excitation wavelengths, demonstrating its use in monitoring light-induced dynamics and Ca2+ signaling.

Oxygen-induced chromophore degradation in the photoswitchable red fluorescent protein rsCherry

The paper investigates oxygen-induced chromophore degradation in rsCherry, identifying cleavage of the p-hydroxyphenyl ring and formation of cyclic structures as the cause of fluorescence loss.

Simultaneous multicolor fluorescence imaging using duplication-based PSF splitting

The paper introduces a method to enhance fluorescence imaging by splitting the PSF into multiple copies, enabling simultaneous multicolor imaging with super-resolution and single-molecule capabilities.

Photochromic fluorophores enable imaging of lowly-expressed proteins in the autofluorescent fungus Candida albicans

The paper uses photochromic fluorophores and SOFI to image lowly-expressed proteins in C. albicans despite high background fluorescence.

Self-contained and modular structured illumination microscope

The paper introduces a modular structured illumination microscope (SIM) called HIT-SIM that enhances existing fluorescence microscopes with fast super-resolution imaging capabilities.

Simultaneous readout of multiple FRET pairs using photochromism

The paper presents a method to simultaneously measure multiple FRET pairs using donor photochromism, validated with biosensors for PKA, ERK, and calcium.

Absolute measurement of cellular activities using photochromic single-fluorophore biosensors

The paper introduces PEAQ biosensing, a method using photochromic single-fluorophore biosensors to measure absolute analyte concentrations or activities in live cells.

Integrated structure-function dataset reveals key mechanisms underlying photochromic fluorescent proteins

The paper develops 27 photochromic fluorescent proteins with varied spectroscopic properties and determines their crystal structures to analyze structure-function relationships.

A Modular Approach to Active Focus Stabilization for Fluorescence Microscopy

This paper presents a modular, cost-effective system for detecting and correcting z-drift in fluorescence microscopy, demonstrating its effectiveness through temperature perturbation and simulated stage movement tests.

Actuation Enhances Patterning in Human Neural Tube Organoids

The paper shows that applying active mechanical forces to human neural tube organoids enhances their patterning and growth through temporally restricted mechanoregulation.

Ultra-Low Colcemid Doses Induce Microtubule Dysfunction as Revealed by Super-Resolution Microscopy

This study uses super-resolution microscopy to show that ultra-low doses of colcemid cause microtubule dysfunction, including curvature and dynamics suppression, at nanomolar concentrations.

SOFIevaluator: a strategy for the quantitative quality assessment of SOFI data

SOFIevaluator quantitatively assesses SOFI data quality and identifies effective fluorescent proteins for SOFI imaging.

High speed multi-plane super-resolution structured illumination microscopy of living cells using an image-splitting prism

This paper introduces a multi-plane SR-SIM technique to enable fast 3D live cell imaging using an image-splitting prism and additional camera.

Quantitative comparison of camera technologies for cost-effective Super-resolution Optical Fluctuation Imaging (SOFI)

This study compares camera technologies for cost-effective Super-resolution Optical Fluctuation Imaging (SOFI), showing that industrial-grade detectors can achieve SOFI on standard and home-built microscopes.

Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM

This paper identifies a long-lived dark state in mEos4b that disrupts sptPALM tracks and shows that 488-nm light can revert it, improving track length and reducing interruptions.