Author

S. J. Royle

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

Click to filter author papers

Cell Biology· 11 Biochemistry· 1

Signature Assays, Organisms & Methods

Click to filter · or open tag

Approach:experimental assay· 10 Research Focus:cell signaling & trafficking· 7 Organism:human· 7 Research Focus:cell type annotation· 4 Assay:imaging· 3 Assay:proteomics· 3 Approach:microscopy & imaging analysis· 2 Research Focus:protein folding & dynamics· 2

Frequent Co-Authors

G. Larocque· 5 shared N. I. Clarke· 5 shared G. P. Starling· 2 shared M. Sittewelle· 2 shared P. J. La-Borde· 2 shared A. J. Beckett· 1 shared A. M. Johansen· 1 shared A. M. Roseman· 1 shared

Activity by Year

2016-2023 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 13 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

5 shared papersCell Biology

G. Larocque

Mutual co-authors in cluster

Author profile →

12 papers

Recent·Most cited

Passive diffusion accounts for the majority of intracellular vesicle transport

M. Sittewelle, S. J. Royle

The paper shows that small intracellular vesicles primarily use passive diffusion for transport, contrasting with motor-driven movement of larger vesicles.

bioRxiv · May 10, 2023Cell Biology

Recruitment of clathrin to intracellular membranes is sufficient for vesicle formation

C. Küey, M. Sittewelle, G. Larocque, M. Hernandez-Gonzalez, S. J. Royle

This study shows that clathrin recruitment alone is sufficient for forming intracellular clathrin-coated vesicles, bypassing the need for accessory proteins.

bioRxiv · Mar 21, 2022Cell Biology

Multi-modal adaptor-clathrin contacts drive coated vesicleassembly

S. M. Smith, G. Larocque, K. M. Wood, K. L. Morris, A. M. Roseman, R. B. Sessions, S. J. Royle, C. J. Smith

This study identifies multi-modal adaptor-clathrin interactions essential for coated vesicle assembly, revealing two critical binding sites on AP2's β2 subunit.

bioRxiv · May 22, 2021Biochemistry

Endomembranes promote chromosome missegregation by ensheathing misaligned chromosomes

N. Ferrandiz, L. Downie, G. P. Starling, S. J. Royle

This study shows that endomembranes ensheath misaligned chromosomes outside the spindle's exclusion zone, delaying mitosis and increasing missegregation, which contributes to aneuploidy.

bioRxiv · Apr 23, 2021Cell Biology

Intracellular nanovesicles mediate integrin trafficking during cell migration

G. Larocque, P. J. La-Borde, B. J. Wilson, N. I. Clarke, D. J. Moore, P. T. Caswell, S. J. Royle

This study identifies intracellular nanovesicles (INVs) as mediators of integrin trafficking during cell migration, showing that TPD52-like proteins regulate this process and affect cell migration and invasion.

bioRxiv · Aug 19, 2020Cell Biology

Defining endogenous TACC3-chTOG-clathrin-GTSE1 interactions at the mitotic spindle using induced relocalization

E. L. Ryan, J. Shelford, T. Massam-Wu, R. Bayliss, S. J. Royle

This paper identifies core and ancillary members of the TACC3-chTOG-clathrin-GTSE1 complex and shows PIK3C2A is not part of the complex, suggesting strategies to disrupt mitotic spindle stability.

bioRxiv · Jul 1, 2020Cell Biology

Tumor Protein D54 defines a new class of intracellular transport vesicle

G. Larocque, P. J. La-Borde, N. I. Clarke, N. J. Carter, S. J. Royle

The paper identifies TPD54 as defining a new class of intracellular transport vesicles called INVs, which have specific cargo, R-SNAREs, and Rab GTPases.

bioRxiv · Aug 12, 2019Cell Biology

Unintended inhibition of protein function using GFP nanobodies in human cells

C. Kuey, G. Larocque, N. I. Clarke, S. J. Royle

The paper demonstrates that GFP nanobodies can inhibit protein function in human cells, limiting their use for knocksideways experiments.

bioRxiv · May 30, 2019Cell Biology

Calculating the maximum capacity of intracellular transport vesicles

E. Martins Ratamero, S. J. Royle

The paper calculates the maximum number of cargo molecules that can fit in vesicles by solving the Tammes Problem for various vesicle and cargo sizes.

bioRxiv · Feb 20, 2019 · PreprintCell Biology

FerriTag: A Genetically-Encoded Inducible Tag for Correlative Light-Electron Microscopy

N. I. Clarke, S. J. Royle

This paper introduces FerriTag, a genetically-encoded inducible tag for correlative light-electron microscopy, demonstrating its utility in labeling and visualizing proteins at nanoscale resolution.

bioRxiv · Dec 18, 2016Cell Biology

Microtubule organization within mitotic spindles revealed by serial block face scanning EM and image analysis

F. M. Nixon, T. R. Honnor, G. P. Starling, A. J. Beckett, A. M. Johansen, J. A. Brettschneider, I. A. Prior, S. J. Royle

The paper describes a correlative light-SBF-SEM workflow to analyze microtubule organization in mitotic spindles and introduces computational tools to study their spatial arrangement.

bioRxiv · Nov 15, 2016Cell Biology

Hot-wiring clathrin-mediated endocytosis in human cells

L. A. Wood, N. I. Clarke, S. Sarkar, S. J. Royle

This paper introduces a method to induce clathrin-mediated endocytosis on demand in human cells and uses it to study interactions between clathrin and the β2 subunit of the AP2 complex.

bioRxiv · Jul 4, 2016Cell Biology