Variations in cell-surface ACE2 levels alter direct binding of SARS-CoV-2 Spike protein and viral infectivity: Implications for measuring Spike protein interactions with animal ACE2 orthologs
S. Kazemi, A. D. Lopez-Munoz, J. Holly, L. Jin, J. W. Yewdell, B. P. Dolan
This paper develops a cell-based assay to measure SARS-CoV-2 Spike protein binding and infectivity across ACE2 orthologs by normalizing ACE2 expression levels using a reporter protein.
bioRxiv · Oct 21, 2021Microbiology