Complementing 16S rRNA gene amplicon sequencing with estimates of total bacterial load to infer absolute species concentrations in the vaginal microbiome
F. Tettamanti Boshier, S. Srinivasan, A. Lopez, N. G. Hoffman, S. Proll, D. N. Fredricks, J. T. Schiffer
The paper combines 16S rRNA gene amplicon sequencing with total bacterial load estimates to infer absolute species concentrations in the vaginal microbiome, showing good correlation with targeted qPCR for relative abundances above 10%.
bioRxiv · Apr 5, 2019Microbiology