Liposome Encapsulation Enables Near-Native Cryo-EM Structural Determination by Shielding Macromolecules from Non-Physiological Interfaces
Gao Zikai, MA Li-juan, Hang Fu, Chenguang Yang, Ma Dongfei, Zhu Hongtao, Yumei Wang, Sun Dapeng, Shuxin Hu, Chunhua Xu, Wei Zhong Ding, Jianbing Ma, Ying Lü, Ming Li
This paper introduces a method using liposome encapsulation to improve cryo-EM structural determination by preserving macromolecules in a near-native environment, reducing disassembly and orientation artifacts.
bioRxiv · May 6, 2025 · PreprintBiophysics