U. Muh

Identification of a new family of peptidoglycan transpeptidases reveals atypical crosslinking is essential for viability in Clostridioides difficile

This study identifies a new family of LDTs in C. difficile that catalyze 3-3 peptidoglycan crosslinking, essential for viability and distinct from previously known YkuD domain LDTs.

The WalRK Two-component system is Essential for Proper Cell Envelope Biogenesis in Clostridioides difficile

This study investigates the essential WalRK two-component system in C. difficile, revealing its role in cell envelope biogenesis and identifying gene expression changes linked to its regulation.

Lysozyme resistance in C. difficile is dependent on two peptidoglycan deacetylases

This paper identifies two peptidoglycan deacetylases, PgdA and PdaV, as essential for lysozyme resistance in C. difficile, showing they are partially redundant and critical for this resistance mechanism.

Activation of the Extracytoplasmic Function σ factor σP by β-lactams in Bacillus thuringiensis requires the site-2 protease RasP

This paper shows β-lactam antibiotics activate σP in Bacillus thuringiensis by degrading the anti-σ factor RsiP through a proteolytic cascade involving site-2 protease RasP.

A xylose-inducible expression system and a CRISPRi-plasmid for targeted knock-down of gene expression in Clostridioides difficile

This paper introduces a xylose-inducible expression system and a CRISPRi plasmid for gene knockdown in Clostridioides difficile, demonstrating their utility in studying gene function.