Site-specific integration of transposon via engineered piggyBac transposase
Naiqing Xu, Lei Han, Xiaohan Hu, Yuan Fang, Limeng Wu, Xi Wang, Haoyang Tu, Xiaogang Deng, Weili Cong, Kailiang Sun, Yi Jin, Xiaoyang Wu
This paper demonstrates a method using engineered piggyBac transposase and CRISPR/Cas9 to achieve targeted integration of large DNA fragments into the human genome with high specificity and efficiency.
bioRxiv · Jan 21, 2026 · PreprintGenetics