A Deep Learning approach for time-consistent cell cycle phase prediction from microscopy data

Abstract

The cell cycle consists of four phases and impacts most cellular processes. In imaging assays, the cycle phase can be identified using dedicated cell-cycle markers. However, such markers occupy fluorescent channels that may be needed for other reporters. Here, we propose to address this limitation by inferring the phase from a widely used fluorescent reporter: SiR-DNA. Our method is based on a variational auto-encoder, enhanced with two auxiliary tasks: predicting the intensity of phase-specific markers and enforcing the latent space temporal consistency. Our model is freely available, along with a new dataset comprising over 600,000 annotated HeLa Kyoto nuclear images.