NAR Genomics and BioinformaticsBioinformaticsPeer-reviewed journal article
mRNAid, an Open-Source Platform for Therapeutic mRNA Design and Optimization Strategies
Nikita Vostrosablin, Shuhui Lim, Pooja GopaliD, Kveta BrazdilovaiD, Sushmita Parajuli, Xiaona Wei, Anna Gromek, David PříhodaiD, Martin SpaleiD, Anja MuždaloiD, Jamie Greig, Constance Yeo, Joanna D. Wardyn, Petr Mejzlík, Brian HenryiD, Anthony W. PartridgeiD, Danny A. BittoniD
Abstract
Recent COVID-19 vaccines unleashed the potential of mRNA-based therapeutics. mRNA optimization is indispensable for reducing immunogenicity, ensuring stability, and maximizing protein output. We present mRNAid, an experimentally validated software for mRNA optimization and visualization that generates mRNA sequences with comparable if not superior characteristics to commercially optimized sequences. To encompass all aspects of mRNA design, we also interrogated the impact of uridine content, nucleoside analogs and UTRs on expression and immunogenicity.
- Citations
- 27
- FWCI
- 3.68
- Field percentile
- top 5%
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