Cystoliths in Ficus leaves: increasing carbon fixation in saturating light by light scattering off a mineral substrate

Abstract

The manner in which leaves adapt to different light intensities is key for enabling plants to survive in diverse environments and in constantly changing conditions. Many studies have addressed this subject, but little attention has been given to the effect that mineral deposits in leaves can have on photosynthesis. Here we study 6 species of Ficus and investigate how different cystolith configurations affect photosynthesis in both non-saturating and saturating light. We quantified the effect of light scattering by cystoliths on light absorption by measuring chlorophyll fluorescence intensity using microfluorimetry. We complement this by carbon assimilation measurements to directly estimate how light scattering by cystoliths affects the overall photosynthetic process. We show that light waste is reduced when irradiance is on a cystolith compared to cystolith free tissue. Moreover, light is channeled into the center of the leaf where photosynthesis occurs more efficiently than in the outer layers. This, in turn, leads to more efficient CO2 assimilation. We conclude that cystoliths contribute to photosynthesis optimization under saturating light. Cystoliths reduce the wasted portion of absorbed light under saturating irradiance by scattering light into the light-deprived leaf center. The increased efficiency may well provide important benefits to plants that form mineral scatterers.