Innovative Microfluidic and Catalytic Techniques Innovation

An open-source, 3D-printable droplet microfluidics platform with strobe-enhanced microscopy

This paper introduces an open-source, 3D-printable droplet microfluidics platform with stroboscopic microscopy for stable droplet generation and low-flow control.

Integrated microdroplet workflow for high-throughput cell-free transcription in double emulsion picoreactors

The paper introduces an integrated microfluidic workflow for high-throughput cell-free transcription using double emulsion picoreactors, enabling quantitative screening of regulatory sequences without specialized sorting equipment.

Ultrahigh-throughput screening for sialic acid-active enzymes in the microbial genetic diversity

The paper develops a microfluidic screening platform to identify sialic acid-active enzymes in metagenomes, discovering a new aldolase enzyme from garden soil.

Open Digital Bioassays Enabled by Aqueous Two-Phase Microreactor Arrays

The paper introduces OASSIS, an open-format digital bioassay platform that enables sensitive, low-complexity RNA detection for point-of-care testing.

Development of a Microdroplet-Based Functional Genomic Screening pipeline by combination of DNA Nanoflowers and PURExpress Cell-Free Expression

The paper develops a microdroplet-based functional genomic screening pipeline using DNA nanoflowers and cell-free expression to identify DNA-interacting enzymes.

A robust and user-agnostic step-emulsion platform for scalable microgel fabrication

This paper introduces a step-emulsion platform for scalable microgel fabrication that is robust, user-agnostic, and compatible with various materials and viscosities.

Open-source robotic chip-to-plate interface for high-throughput microfluidic generation of materials libraries

LMNOP-bot automates microfluidic material library generation, increasing throughput 50x and enabling precise, reproducible deposition into standard well plates.

Simple and Multiplexed Tracking of Bacterial Growth in Double Emulsion Droplets

This paper introduces a simple method to track bacterial growth in droplets over 12 hours, revealing growth rate heterogeneity not apparent in batch assays.

A droplet microfluidic-based platform for enhanced DNA delivery in non-model organisms

This paper introduces a droplet microfluidic platform that significantly improves DNA delivery efficiency in non-model organisms through enhanced conjugation.

An expedient, biology-laboratory-compatible method for preparing functional perfluoropolyether fluorosurfactants for droplet microfluidics

This paper describes a simple method for preparing functional PFPE-based fluorosurfactants in biology labs, enabling low-cost and customizable droplet microfluidic applications.

Precise loading of scarce reagents on droplet microarrays

This paper introduces a method to precisely load scarce reagents on droplet microarrays, reducing material usage and enabling high-throughput assays with minimal volumes.

DropletFactory CORE - a droplet cytometry and sorting platform for fast and accessible screening in biotechnology

The paper introduces a droplet cytometry and sorting platform for fast and accessible screening of yeast cells based on fluorescence signals.

Scalable flow synthesis of ultrasmall inorganic nanoparticles for biomedical applications via a confined impinging jet mixer

This paper develops a scalable method to produce ultrasmall inorganic nanoparticles for biomedical use using a confined impinging jet mixer.

Fine-tuning cell-mimicking polyacrylamide microgels: Sensitivity to microscale reaction conditions in droplet microfluidics

This study shows that flow conditions in droplet microfluidics significantly affect the elasticity and size of PAAm microgels, despite identical monomer concentrations.

Post-Assay Photogelation Enables Flow-Cytometric Sorting and Recovery of Bacterial Cocultures from Microfluidic Droplets

This paper introduces post-assay photogelation to enable flow-cytometric sorting of bacterial cocultures from microfluidic droplets, achieving high-purity sorting and demonstrating its application in microbial community analysis.

MusTer: A Generalizable Microfluidic Platform Combining Multi-Parametric Droplet Sorting and Multi-Droplet Merging in Single-Cell Sequencing

MusTer improves single-cell sequencing by reducing doublets, enhancing signal-to-noise ratio, and improving cell clustering in maize leaf ATAC-seq.

Automated and parallelized microfluidic generation of large and precisely-defined lipid nanoparticle libraries

This paper presents an automated microfluidic platform for generating large, precisely-defined lipid nanoparticle libraries and evaluates their physicochemical and transfection properties.

Monodisperse LNPs - from Efficient Microfluidic Production and Loading to in Vitro Testing

This paper develops a microfluidic device called LARLM for producing uniform lipid nanoparticles (LNPs) with high productivity and demonstrates improved bioavailability of loaded drugs in vitro.

Ultrahigh-Throughput Forster Resonance Energy Transfer-Activated Droplet Sorting for Terminator Polymerase Engineering

This paper introduces a dual-channel FRET-based droplet sorting system to enhance terminator polymerase engineering, achieving 30-fold enrichment of active variants in a single round.

High-throughput single cell -omics using semi-permeable capsules

This paper introduces a semi-permeable capsule technology for high-throughput single-cell -omics, demonstrating its utility in scRNA-Seq of hematopoietic disorders and uncovering transcriptome changes in AML.

Interfacial Tension Modulates Viscous Microfluidic Droplet Generation

The paper shows that lower interfacial tension, achieved by surfactant concentration, can overcome the challenges of high viscosity in microfluidic droplet generation using basement membrane extract.

Deterministic co-encapsulation of microparticles in droplets via synchronized merging for single-cell genomics

The paper develops a method for deterministic co-encapsulation of microparticles in droplets for single-cell genomics, achieving accurate taxonomic profiling of microbial cells.

Droplet-based microfluidics platform for investigation of protoplast development of three exemplary plant species

This study develops a droplet-based microfluidic platform for culturing plant protoplasts and evaluates its effectiveness across three species, finding tobacco protoplasts show highest viability.

Space-efficient method for high-throughput generation of uniform cell-laden hollow agarose microcapsules

The paper presents a space-efficient method for generating uniform hollow agarose microcapsules using a sealed bottle and vortex mixer, enabling high-throughput encapsulation in confined environments.

Droplet microfluidic PicoSorter for high throughput and active selection of cellulolytic microorganisms.

The paper introduces a microfluidic device called PicoSorter for high-throughput selection of cellulolytic microorganisms using droplet-based assays and sorting.

Precise Control of Sodium Alginate Microdroplets through pH-Sensitive Cross-Linkers and Geometric Factors

This paper develops a method to create uniform sodium alginate microdroplets using pH-sensitive cross-linkers and geometric control, achieving low variation and stable gels.

Induced Droplet Ovalisation (IDO): Image-based microfluidic method for high-throughput and label-free characterization of microbial proteolytic strains from wastewater sludge.

This paper introduces a microfluidic method called IDO for high-throughput, label-free screening of microbial proteolytic activity from wastewater sludge, showing improved efficiency and sensitivity compared to traditional methods.

Continuous FACS sorting of double emulsion picoreactors with a 3D printed vertical mixer.

This paper improves the efficiency of FACS sorting of double emulsion picoreactors by optimizing surfactants and using a 3D-printed vertical mixer, achieving high accuracy and recovery in sorting.

Light-orchestrated multi-step solid-phase picodroplet reactors

This paper introduces a light-controlled picodroplet reactor platform capable of executing multi-step biochemical reactions with precise reagent control and real-time monitoring.

SCO-pH: Microfluidic dynamic phenotyping platform for high-throughput screening of single cell acidification

SCO-pH enables high-throughput single-cell acidification analysis by compartmentalizing cells in microfluidic droplets and measuring extracellular pH changes to distinguish metabolic states.