Discover, rate, and discuss biology preprints.

The paper develops a high-fidelity CRISPR-Cas13d system for RNA depletion in Plasmodium falciparum, achieving significant target mRNA reduction and functional impairment of sexual differentiation.

This study compares tumor-immune-stromal communication in plasmacytoid urothelial carcinoma (PUC) and conventional urothelial carcinoma (CUC) using single nucleus RNA sequencing and spatial transcriptomics, identifying distinct signaling patterns and potential therapeutic targets.

This study examines how CD19 CAR T cell therapy affects gut microbiota and immune responses in autoimmune disease patients, finding changes in microbiota composition and antibody repertoires linked to relapse risk.

The paper identifies acetylcholine as a key mediator of hatching enzyme release in red-eyed treefrog embryos, revealing conserved neural mechanisms across amphibians.

This study identifies early proteomic changes in cardiac fibroblasts after doxorubicin treatment, revealing ECM and mitochondrial-related proteins associated with early senescence responses.

The paper examines methane production and microbial responses to carbon enrichment in freshwater wetland sediments using field and lab experiments.

This study shows that light power density during photodynamic therapy determines systemic antitumor effects by influencing singlet oxygen generation, tumor perfusion, and circulating extracellular vesicles.

This paper introduces stochprofIC, an algorithm for noise-corrected state variability inference in single cells, validated using mini-bulk data from mouse brains and human breast cancers.

This study investigates actin reorganization and junctional remodeling in podocyte foot process effacement using advanced imaging techniques and shows reversible changes in actin and slit diaphragm proteins during nephrotoxic serum nephritis.

The paper shows that SPY-mediated O-fucosylation and BIN2-mediated phosphorylation antagonistically regulate PCK1 activity, affecting metabolism and growth in Arabidopsis.

The paper introduces SEMPER, a multimodal model that aligns protein language models with brain cell-type-specific interactomes to predict protein functions and drug targets.

DM4ISL improves in silico labeling of fluorescence images by reducing noise artifacts and providing uncertainty maps, outperforming existing methods across six organelles.

This study identifies Ksp37 as a rapidly secreted NK cell protein that binds FGF2, suggesting a role in modulating extracellular environment and tumor-related processes.

This study shows sleep restores neuronal autophagy in mice, reducing amyloid-beta accumulation and highlighting the role of brain-state timing in proteostasis.

This study evaluates N8-propargyl spermidine as a chemical probe for hypusination and challenges claims of expanded DHS substrate specificity.

This paper develops an anaerobic cell-free protein synthesis platform to study oxygen-sensitive proteins, demonstrating its ability to produce functional enzymes under anoxic conditions.

The paper designs and tests composite biosensor-gate circuits in soil bacteria to improve environmental synthetic biology applications.

scCody distinguishes aging-aligned from divergent disease-associated cellular remodeling by learning age-dependent dynamics and decomposing disease effects into aging-related and orthogonal components.

The paper identifies RabE and RabA-mediated trafficking as distinct pathways in plant pre-invasive immunity, with RabE specific to powdery mildew and RabA to oomycete pathogens.

The paper reanalyzes proteomic data to identify detection limits for acquired lysines in cancer, finding low recovery rates and high false discovery proportions due to technical challenges.