A. Buschle

Highly efficient CRISPR-Cas9-mediated gene knockout in primary human B cells for functional genetic studies of Epstein-Barr virus infection

Human ORC/MCM density is low in active genes and correlates with replication time but does not solely define replication initiation zones

The paper examines ORC/MCM density in relation to replication timing and initiation zones, finding no direct correlation with initiation but a link to replication timing.

Active transcription regulates ORC/MCM distribution whereas replication timing correlates with ORC density in human cells

The paper compares ChIP-seq profiles of ORC and MCM proteins with replication timing to show that active transcription affects ORC/MCM distribution and replication timing correlates with ORC density in human cells.

Epstein-Barr virus inactivates the transcriptome and disrupts the chromatin architecture of its host cell in the first phase of lytic reactivation

This study shows that EBV lytic reactivation causes global chromatin remodeling and transcriptome disruption in host cells before viral DNA replication.

Epstein-Barr virus reprograms human B-lymphocytes immediately in the pre-latent phase of infection

This study shows EBV rapidly reprograms primary human B-lymphocytes transcriptionally within 24 hours of infection, leading to metabolic changes and cell cycle entry by day three.

BZLF1 interacts with the chromatin remodeler INO80 promoting escape from latent infections with Epstein-Barr virus

This study identifies BZLF1's role in reactivating Epstein-Barr virus by interacting with the chromatin remodeler INO80 to overcome epigenetic silencing.