Structure guided engineering of a cold active esterase expands substrate range though a stabilisation mutation that allows access to a buried water chamber
N. Noby, R. Johnson, J. Tyzack, A. Embaby, H. Saeed, A. Hussein, S. Khattab, P. Rizkallah, D. Jones
The paper engineers a cold-active esterase to expand its substrate range by stabilizing a structural mutation that allows access to a buried water chamber.
bioRxiv · Feb 23, 2021 · PreprintBiochemistry