Long-Term Persistence of Plasmids Targeted by CRISPR Interference in Bacterial Populations
V. Mamontov, A. Martynov, N. Morozova, A. Bukatin, D. B. Staroverov, K. A. Lukyanov, Y. Ispolatov, E. Semenova, K. Severinov
The paper shows plasmids can persist in E. coli under continuous CRISPR targeting without genetic changes, due to stochastic processes and plasmid replication dynamics.
bioRxiv · Apr 3, 2021 · PreprintMicrobiology