Directed evolution of the rRNA methylating enzyme Cfr reveals molecular basis of antibiotic resistance
K. Tsai, V. Stojkovic, L. Noda-Garcia, I. D. Young, A. G. Myasnikov, J. Kleinman, A. Palla, S. N. Floor, A. Frost, J. S. Fraser, D. S. Tawfik, D. Galonic Fujimori
This study uses directed evolution to identify Cfr variants that enhance rRNA methylation, revealing structural insights into antibiotic resistance mechanisms.
bioRxiv · Mar 12, 2021Biochemistry