Author

A. Frost

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 8 Biophysics· 7 Cell Biology· 4 Bioengineering· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 17 Research Focus:cell signaling & trafficking· 11 Assay:cryo-EM· 7 Organism:human· 7 Research Focus:protein folding & dynamics· 6 Research Focus:protein structure prediction· 6 Research Focus:chromatin & epigenetic regulation· 5 Approach:microscopy & imaging analysis· 4

Frequent Co-Authors

H. C. Nguyen· 4 shared P. Walter· 4 shared F. R. Moss· 3 shared L. E. Miller-Vedam· 3 shared R. Kalia· 3 shared A. A. Anand· 2 shared A. G. Myasnikov· 2 shared A. Sharma· 2 shared

Activity by Year

2016-2023 · peak 6/yr

Co-Author Collaboration Network

10 top collaborators · 14 mutual co-author links

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4 shared papersBiophysics

H. C. Nguyen

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Author profile →

20 papers

Recent·Most cited

Structural basis of TRPV1 modulation by endogenous bioactive lipids

W. R. Arnold, A. S. Mancino, F. R. Moss, A. Frost, D. Julius, Y. Cheng

This study identifies a lipid-binding pocket in TRPV1 and shows that its occupancy by phosphoinositides modulates channel activity, with lipid ejection triggering activation.

bioRxiv · May 11, 2023Biochemistry

A structural biology community assessment of AlphaFold 2 applications

M. Akdel, D. E. Pires, E. Porta-Pardo, J. Janes, A. O. Zalevsky, B. Meszaros, P. Bryant, L. L. Good, R. A. Laskowski, G. Pozzati, A. Shenoy, W. Zhu, P. Kundrotas, V. Ruiz-Serra, C. H. Rodrigues, , P. Beltrao

This study assesses AlphaFold 2's applications in structural biology, finding it outperforms existing tools in predicting disorder, complexes, and structural features with high confidence.

bioRxiv · Sep 26, 2021 · PreprintBiophysics

Brominated Lipid Probes Expose Structural Asymmetries in Constricted Membranes

F. R. Moss, J. Lincoff, M. Tucker, A. Mohammed, M. Grabe, A. Frost

The paper uses brominated lipids and cryo-EM to reveal structural asymmetries in constricted membranes.

bioRxiv · Aug 24, 2021Biophysics

Viral Evasion of the Integrated Stress Response Through Antagonistic eIF2-P Mimicry

M. Schoof, L. Wang, J. Z. Cogan, R. Lawrence, M. Boone, J. D. Wuerth, A. Frost, P. Walter

This study shows that SFSV NSs protein evades the ISR by competing with eIF2-P for binding to eIF2B, preserving its activity and allowing viral protein synthesis.

bioRxiv · Jun 7, 2021Biochemistry

Directed evolution of the rRNA methylating enzyme Cfr reveals molecular basis of antibiotic resistance

K. Tsai, V. Stojkovic, L. Noda-Garcia, I. D. Young, A. G. Myasnikov, J. Kleinman, A. Palla, S. N. Floor, A. Frost, J. S. Fraser, D. S. Tawfik, D. Galonic Fujimori

This study uses directed evolution to identify Cfr variants that enhance rRNA methylation, revealing structural insights into antibiotic resistance mechanisms.

bioRxiv · Mar 12, 2021Biochemistry

eIF2B Conformation and Assembly State Regulate the Integrated Stress Response

M. Schoof, M. Boone, L. Wang, R. E. Lawrence, A. Frost, P. Walter

This study shows that eIF2B assembly and conformation regulate the ISR, with ISRIB affecting eIF2B through decamer stabilization and allosteric modulation.

bioRxiv · Dec 28, 2020Cell Biology

Practical considerations for using K3 cameras in CDS mode for high-resolution and high-throughput single particle cryo-EM

M. Sun, C. M. Azumaya, E. Tse, A. Frost, D. Southworth, K. A. Verba, Y. Cheng, D. A. Agard

This study optimizes K3 camera settings in CDS mode for cryo-EM, showing improved resolution and throughput for protein complex imaging.

bioRxiv · Nov 8, 2020Biophysics

Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clients

L. E. Miller-Vedam, B. Bräuning, K. D. Popova, N. T. Schirle Oakdale, J. L. Bonnar, J. R. Prabu, E. A. Boydston, N. Sevillano, M. J. Shurtleff, R. M. Stroud, C. S. Craik, B. Schulman, A. Frost, J. S. Weissman

The paper reveals the structural and mechanistic basis of EMC's role in biogenesis of distinct transmembrane proteins through cryo-EM structures and functional studies.

bioRxiv · Sep 2, 2020Biophysics

Photocatalytic plant LPOR forms helical lattices that shape membranes for chlorophyll synthesis

H. C. Nguyen, A. A. Melo, J. Kruk, A. Frost, M. Gabruk

This study reveals the atomic structure of LPOR assemblies and shows how they shape membranes and catalyze chlorophyll synthesis in plants.

bioRxiv · Aug 19, 2020Biophysics

Membrane constriction and thinning by sequential ESCRT-III polymerization

H. C. Nguyen, N. Talledge, J. McCullough, A. Sharma, F. R. Moss, J. H. Iwasa, M. D. Vershinin, W. I. Sundquist, A. Frost

This study determines the structures of ESCRT-III filaments to show how sequential polymerization by CHMP1B and IST1 drives membrane tubulation, constriction, and thinning.

bioRxiv · Oct 11, 2019Biophysics

Anisotropic ESCRT-III architecture governs helical membrane tube formation

J. Moser von Filseck, L. Barberi, N. Talledge, I. Johnson, A. Frost, M. Lenz, A. Roux

The paper describes how ESCRT-III filaments assemble into helical membrane tubes through anisotropic architecture and mathematical modeling.

bioRxiv · Jul 26, 2019Biochemistry

High-resolution cryo-electron microscopy structure of the Escherichia coli 50S subunit and validation of nucleotide modifications

V. Stojkovic, A. Myasnikov, I. Young, A. Frost, J. Fraser, D. Fujmori

The paper presents a high-resolution cryo-EM structure of E. coli 50S subunit and develops a program for identifying rRNA modifications using cryo-EM maps.

bioRxiv · Jul 7, 2019 · PreprintBiochemistry

The mechanisms of dynamin-actin interaction

R. Zhang, N. Gerassimov, D. M. Lee, J. R. Jimah, S. Kim, D. Luvsanjav, J. Winkelman, M. Mettlen, M. E. Abrams, R. Kalia, P. Keene, P. Pandey, B. Ravaux, J. H. Kim, J. A. Ditlev, , E. H. Chen

This study identifies a noncanonical role for dynamin in cell-cell fusion by directly bundling actin filaments through GTPase-dependent interactions.

bioRxiv · Mar 25, 2019 · PreprintCell Biology

A role for liquid-liquid phase separation in ESCRT-mediated nuclear envelope reformation.

A. von Appen, D. Lajoie, I. E. Johnson, M. Trnka, S. M. Pick, A. L. Burlingame, K. S. Ullman, A. Frost

This study shows how LEM2 uses liquid-liquid phase separation and ESCRT pathways to facilitate nuclear envelope reformation during mitotic exit.

bioRxiv · Mar 14, 2019Cell Biology

A tunable microfluidic device enables cargo encapsulation by cell- or organelle-sized lipid vesicles comprising asymmetric lipid monolayers

V. Romanov, J. McCullough, A. Sharma, M. Vershinin, B. K. Gale, A. Frost

This paper introduces a microfluidic device for creating lipid vesicles with tunable sizes and asymmetric lipid monolayers, capable of encapsulating cargo and supporting membrane processes.

bioRxiv · Jan 29, 2019Bioengineering

Structural basis of eIF2B-catalyzed GDP exchange and phosphoregulation by the integrated stress response

A. A. Anand, L. R. Kenner, H. C. Nguyen, A. G. Myasnikov, C. J. Klose, L. A. McGeever, J. C. Tsai, L. E. Miller-Vedam, P. Walter, A. Frost

The paper reports cryoEM structures of eIF2 bound to eIF2B, revealing the mechanism of GDP exchange and phosphoregulation in the integrated stress response.

bioRxiv · Dec 22, 2018Biochemistry

Vms1p is a release factor for the Ribosome-associated Quality control Complex

O. Z. Rendon, E. K. Fredrickson, C. J. Howard, J. V. Vranken, S. Fogarty, N. D. Tolley, R. Kalia, B. A. Osuna, P. S. Shen, C. P. Hill, A. Frost, J. Rutter

This study identifies Vms1p as the tRNA hydrolase that releases nascent polypeptides in the RQC pathway for degradation.

bioRxiv · Apr 14, 2018Biochemistry

Structure of the nucleotide exchange factor eIF2B reveals mechanism of memory-enhancing molecule

J. C. Tsai, L. E. Miller-Vedam, A. A. Anand, P. Jaishankar, H. C. Nguyen, A. R. Renslo, A. Frost, P. Walter

The paper determines the structure of eIF2B bound to ISRIB, revealing how it promotes eIF2B assembly and reverses eIF2 phosphorylation effects.

bioRxiv · Nov 24, 2017Biochemistry

Structural Basis of Mitochondrial Receptor Binding and GTP Driven Conformational Constriction by Dynamin-Related Protein 1

R. Kalia, R. Y.- R. Wang, A. Yusuf, P. V. Thomas, D. A. Agard, J. M. Shaw, A. Frost

This study presents cryoEM structures of DRP1 bound to MID49 and nucleotides, revealing how GTP binding drives conformational changes essential for mitochondrial fission.

bioRxiv · Aug 7, 2017Biophysics

LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells

M. Gu, D. LaJoie, O. S. Chen, A. Von Appen, M. S. Ladinsky, M. J. Redd, L. Nikolova, P. J. Bjorkman, W. I. Sundquist, K. S. Ullman, A. Frost

This study identifies LEM2 and CHMP7 as conserved factors that recruit ESCRT-III proteins to the nuclear envelope during closure in fission yeast and human cells.

bioRxiv · Apr 19, 2016Cell Biology