CRISPR-Cas12a-assisted PCR tagging of mammalian genes
J. Fueller, K. Herbst, M. Meurer, K. Gubicza, B. Kurtulmus, J. D. Knopf, D. Kirrmaier, B. Buchmuller, G. Pereira, M. K. Lemberg, M. Knop
This paper describes a method for efficient endogenous gene tagging in mammalian cells using CRISPR-Cas12a and PCR cassettes, achieving up to 60% tagging efficiency with selection.
Journal of Cell Biology · May 13, 2020Peer-reviewedCell Biology