BiochemistrybioRxiv
Fluorescence-Detected Conformational Changes in Duplex DNA in Open Complex Formation by E. coli RNA Polymerase: Upstream Wrapping and Downstream Bending Precede Clamp Opening and Insertion of the Downstream Duplex
R. Sreenivasan, I. A. Shkel, M. Chhabra…·M. T. Record
This study uses FRET and PIFE to analyze DNA conformational changes during E. coli RNA polymerase open complex formation, revealing upstream wrapping and downstream bending before clamp opening.
DOI 10.1021/acs.biochem.0c00098