Fluorescence-Detected Conformational Changes in Duplex DNA in Open Complex Formation by E. coli RNA Polymerase: Upstream Wrapping and Downstream Bending Precede Clamp Opening and Insertion of the Downstream Duplex
R. Sreenivasan, I. A. Shkel, M. Chhabra, A. Drennan, S. Heitkamp, H.-C. Wang, M. A. Sridevi, D. Plaskon, C. McNerney, K. Callies, C. K. Cimperman, M. T. Record
This study uses FRET and PIFE to analyze DNA conformational changes during E. coli RNA polymerase open complex formation, revealing upstream wrapping and downstream bending before clamp opening.
bioRxiv · Feb 4, 2020Biochemistry