Dynamic, single-cell monitoring of CAR T cell identity and activation with Raman spectroscopy
Ariel Stiber, Boi Bryant Quach, Babatunde Ogunlade, Antony Georgiadis, Kai Chang, Y. Li, Patrick J. Quinn, Haoqing Wang, Kristin CY Tsui, Charm Ang, Elena Sotillo, David Bernard Miklos, Crystal Lee Mackall, Zinaida Good, Jennifer A. Dionne
Gold-nanorod surface-enhanced Raman spectroscopy with LightGBM separates CAR T from untransduced T cells, tracks antigen-specific activation dynamics, and discriminates sorted CAR+ from CAR- patient T cells.
bioRxiv · Jul 18, 2026 · PreprintBioengineering✦ In-depth digest