Christopher Mullen

Unifying N- and O-glycopeptide dissociation methods with expedient electron transfer dissociation

This paper improves ETD-based fragmentation for glycopeptide analysis by optimizing ion handling and scan parameters, making it faster and more efficient for both N- and O-glycopeptides.

Beyond HCD: complementary fragmentation chemistries expand HLA class I immunopeptidome discovery

This paper evaluates CID and IRMPD fragmentation methods for HLA class I immunopeptidomics, finding they identify unique peptides missed by HCD, especially for non-basic anchor alleles and peptides lacking lysine, arginine, or histidine.

Defining glycoproteoform landscapes through an integrated glycoproteomics approach enabled by high-resolving power proton transfer charge reduction tandem mass spectrometry

This paper introduces a high-resolving power DIA-PTCR method for glycoproteoform analysis, enabling isotopically resolved mass measurements and integration with other glycoproteomics approaches.

Defining state-selective lipid binding to brain GPCRs - introducing REVEAL

The paper introduces REVEAL, a mass spectrometry method to identify receptor-specific lipid binding in mammalian membrane proteins, showing agonism alters lipid occupancy and plasmalogen lipids are depleted under oxidative stress.

Native Mass Spectrometry-Based Proteomics Reveals the Mechanism of Hemophore Release by Pathogenic Corynebacterium diphtheriae

This study introduces ProteoMIX, a native top-down proteomics method to identify hemophores in C. diphtheriae, revealing protease-mediated release of soluble heme-binding proteoforms.