Ralf B. Schittenhelm

This paper evaluates CID and IRMPD fragmentation methods for HLA class I immunopeptidomics, finding they identify unique peptides missed by HCD, especially for non-basic anchor alleles and peptides lacking lysine, arginine, or histidine.

This paper describes a method to simultaneously profile the transcriptome and proteome from single cells, showing moderate correlation between the two modalities.

This study shows that stress reveals impaired endocrine coordination of glucose metabolism in dystrophin deficiency, with interventions improving glucose control and muscle function.

The paper identifies temporal expression patterns of liver-stage malaria antigens and shows that combining antigens with different temporal profiles improves vaccine efficacy.

The paper maps the chemokine-stimulated signal transduction network in monocytic cells and shows that chemotaxis emerges from a divergent signaling network that can be inhibited to block cell migration.

The paper shows ELP3 modifies tRNA at U34 and regulates proteome composition through specific di-codon pairs, affecting cancer cell proliferation.

The paper benchmarks plasma proteomic workflows across species and methods, showing workflow choice significantly impacts detected proteins and depth.

The paper shows that catabolite repression regulates hydrogen consumption in Mycobacterium smegmatis by modulating the expression of the huc operon in response to glycerol availability.

This study validates solvent proteome profiling (SPP) as a method to identify antimalarial drug targets in Plasmodium falciparum by detecting ligand-induced protein stability changes.

This study shows that the AML chemotherapy regimen induces cachexia in mice, characterized by body and lean mass loss, and identifies haptoglobin as a potential biomarker for chemotherapy-induced cachexia.

This study identifies six immunogenic peptides in diffuse midline glioma (DMG) linked to the H3K27M mutation, offering potential targets for T cell-based immunotherapy.

The paper shows ribosomal synthesis generates a noncanonical 3-nitrotyrosine building block used in nonribosomal peptide synthesis of rufomycin.

This study identifies ribosomal biogenesis hyperactivation and ErbB signaling reactivation as mechanisms of resistance to FGFR2 inhibitors in breast cancer cells and shows synergy with MEK inhibitors.

The paper presents the structure of Tse15, a T6SS Rhs effector from Acinetobacter baumannii, revealing a novel mechanism for toxin delivery and activation involving auto-cleavage and domain interaction.

FragPipe-Analyst provides an R shiny web server and R package for downstream analysis and visualization of quantitative proteomics data from FragPipe.

This study establishes a Drosophila model of ECHS1 deficiency and shows that valine restriction improves larval survival.

This study integrates multi-OMIC data from 12 cohorts to identify exercise-specific molecular signatures and gene regulatory patterns in human skeletal muscle.

This study shows that estrogen loss in female mdx mice reduces exercise capacity and mitochondrial metabolism, but estrogen replacement therapy can reverse these effects and upregulate Rbm20.

This paper introduces an adjusted diet formula for Drosophila melanogaster larvae to improve growth, development, and survival, with applications in studying metabolic disorders.

This study shows GCN2-mediated autophagy allows Drosophila to use stored amino acids for somatic maintenance during dietary deprivation, extending lifespan.

This study uses human kidney organoids to model hypoxic injury and maladaptive repair, identifying molecular and metabolic changes associated with AKI and CKD progression.

This study reveals how Mycobacterium smegmatis adapts to hypoxia by combining fermentative hydrogen production with carbon storage through lipid droplets.

This study shows sodium selenate reduces seizures, cognitive dysfunction, and sensorimotor deficits in a rat model of chronic drug-resistant temporal lobe epilepsy.

The paper uses a zebrafish model of MPS IIIA to identify synaptic activity deficiencies as a key driver of disease progression through systems-level omics analyses and a novel bioinformatic tool.

This study integrates DNA methylation and proteomics data from human skeletal muscle to identify group and individual responses to high-intensity interval training.

This study shows that carbon monoxide dehydrogenases enhance bacterial survival by providing a supplemental energy source during organic carbon starvation.