Author

D. A. Cisneros

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Microbiology· 2

Signature Assays, Organisms & Methods

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Research Focus:gene regulatory network inference· 2 Organism:bacteria (other)· 1 Research Focus:clustering· 1 Approach:deep learning· 1 Research Focus:differential expression· 1 Assay:DNA methylation· 1 Organism:E. coli· 1 Approach:experimental assay· 1

Frequent Co-Authors

A. Graffeuil· 1 shared A. Puhar· 1 shared A. Sharma· 1 shared B. E. Uhlin· 1 shared R. Omer Aden· 1 shared

Activity by Year

2020-2022 · peak 1/yr

Co-Author Collaboration Network

5 top collaborators · 4 mutual co-author links

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1 shared paperMicrobiology

A. Graffeuil

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2 papers

Recent·Most cited

CRISPR-Cas guided mutagenesis of chromosome and virulence plasmid in Shigella flexneri by cytosine base editing

A. Sharma, R. Omer Aden, A. Puhar, D. A. Cisneros

This paper develops a CRISPR-Cas base editing method to generate targeted loss-of-function mutants in Shigella flexneri with high efficacy and throughput.

bioRxiv · Mar 4, 2022Microbiology

Polar mutagenesis of bacterial transcriptional units using Cas12a

A. Graffeuil, B. E. Uhlin, D. A. Cisneros

The paper demonstrates that Cas12a can efficiently engineer polar mutations in the fim operon of E. coli, disrupting gene expression and showing potential for transcriptional shutdown in metabolic engineering.

bioRxiv · Feb 21, 2020 · PreprintMicrobiology