Bioluminescence-based reporters for characterizing inhibitors and activators of human Sonic Hedgehog protein autoprocessing in live cells at high throughput.
D. Ciulla, P. Dranchak, J. Pezzullo, R. Mancusi, A. Psaras, G. Rai, J.-L. Giner, J. Inglese, B. Callahan
This paper develops a bioluminescence-based reporter system to monitor human SHhC autoprocessing in live cells for high-throughput screening of inhibitors and activators.
bioRxiv · Jun 27, 2022Biochemistry