High-quality ultrastructural preservation using cryofixation for 3D electron microscopy of genetically labeled tissues
T. K. Tsang, E. A. Bushong, D. Boassa, J. Hu, B. Romoli, S. Phan, D. Dulcis, C.-Y. Su, M. H. Ellisman
This paper develops a method to enable genetically labeled cryofixed samples to be analyzed with SBEM and 3D CLEM, overcoming previous technical limitations.
bioRxiv · Feb 7, 2018Biophysics