An optimised method for bacterial nucleic acid extraction from positive blood culture broths for whole genome sequencing, resistance phenotype prediction and downstream molecular applications
M. Bauer, A. M. Peri, L. Luftinger, S. Beisken, H. Bergh, B. M. Forde, C. Buckley, T. Cuddihy, P. Tan, D. Paterson, D. M. Whiley, P. N. A. Harris
This paper optimizes DNA extraction from blood culture broths for whole genome sequencing and resistance prediction, showing high accuracy for common pathogens and potential for rapid diagnostics.
bioRxiv · Jun 30, 2022 · PreprintMicrobiology