Nucleotide binding halts diffusion of the eukaryotic replicative helicase during activation
D. Ramirez Montero, H. Sanchez, E. van Veen, T. van Laar, B. Solano, J. F. X. Diffley, N. H. Dekker
This study reports the first single-molecule activation of CMG reconstituted from purified yeast proteins and quantifies its DNA motion, showing nucleotide binding halts diffusion and enables DNA melting.
bioRxiv · Dec 23, 2022Biophysics