E. J. Strobel

Systematic analysis of cotranscriptional RNA folding using transcription elongation complex display

This study introduces TECdisplay, a high-throughput method for analyzing cotranscriptional RNA folding, and applies it to study riboswitch function.

Purification of synchronized E. coli transcription elongation complexes by reversible immobilization on magnetic beads

This paper develops a method to purify synchronized E. coli transcription elongation complexes using a custom leader sequence, enabling high-purity, active, and synchronous TECs for RNA assays.

Efficient linear dsDNA tagging using deoxyuridine excision

This paper introduces a method for efficient linear dsDNA tagging using dU excision, enabling high-efficiency modification with commercially available reagents.

Preparation of E. coli RNA polymerase transcription elongation complexes for systematic RNA assays

The paper develops a method to purify E. coli RNA polymerase transcription elongation complexes for systematic RNA sequence-function studies.

Chemical transcription roadblocking for nascent RNA display

This paper introduces a chemical method to block RNA polymerase transcription in E. coli using DNA modifications, enabling stable nascent RNA display in minimal in vitro reactions.

A mechanism for ligand gated strand displacement in ZTP riboswitch transcription regulation

This study identifies the cotranscriptional folding pathway and regulatory mechanism of the Clostridium beijerinckii pfl riboswitch in response to ZMP.

Computationally Reconstructing Cotranscriptional RNA Folding Pathways from Experimental Data Reveals Rearrangement of Non-Native Folding Intermediates

This paper introduces R2D2, a method to predict RNA folding pathways from cotranscriptional SHAPE-Seq data, revealing non-native intermediates in E. coli SRP RNA folding.