Conformational Ensembles Reveal the Origins of Serine Protease Catalysis
S. Du, R. C. Kretsch, J. Parres-Gold, E. Pieri, V. W. D. Cruzeiro, M. Zhu, M. M. Pinney, F. Yabukarski, J. P. Schwans, T. J. Martinez, D. Herschlag
Ensemble-function analyses provide a quantitative model for serine protease catalysis, reveal previously unknown conformational features that contribute to their catalysis, and identify a structural motif that underlie these features and has evolved in >100 different enzymes from 32 protein folds.
bioRxiv · Feb 28, 2024Biophysics