E. Rembeza

Experimental investigation of enzyme functional annotations reveals extensive annotation error

The paper experimentally investigates functional annotations in the BRENDA database, finding that 78% of S-2-hydroxyacid oxidases are misannotated and 18% of all sequences are annotated without similarity to characterized representatives.

Adaptation of a microfluidic qPCR system for enzyme kinetics studies

The paper adapts a commercial microfluidic qPCR system for enzyme kinetics studies, showing reproducible results comparable to standard microplate assays.

Structural basis of human 5,10-methylenetetrahydrofolate reductase (MTHFR) regulation by phosphorylation and S-adenosylmethionine inhibition

This study presents the 2.5 Å resolution crystal structure of human MTHFR, revealing its architecture and how phosphorylation and SAM binding regulate its activity.