How Glutamate Promotes Liquid-liquid Phase Separation and DNA Binding Cooperativity of E. coli SSB Protein
A. G. Kozlov, X. Cheng, H. Zhang, M. K. Shinn, E. Weiland, B. Nguyen, I. A. Shkel, E. Zytkiewicz, I. J. Finkelstein, M. T. Record, T. M. Lohman
This study shows that potassium glutamate promotes liquid-liquid phase separation and non-nearest-neighbor cooperativity of E. coli SSB protein through amide group interactions, while KCl inhibits these processes.
bioRxiv · Jan 17, 2022Biochemistry