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Emi Nakai

Hiroshima Institute of Technology

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Synthetic Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 1 Research Focus:protein design· 1 Assay:protein structure· 1 Research Focus:variant effect prediction· 1

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Naoki Takami· 1 shared Tadashi NakaiiD· 1 shared Toshihide OkajimaiD· 1 shared Yota Nakai· 1 shared

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1 shared paperSynthetic Biology

Naoki Takami

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Synthetic BiologyACS OmegaPeer-reviewedJul 25, 2025

SpyRing-Mediated Cyclization of TEV Protease, Guided by AlphaFold, Improves Thermostability

Tadashi Nakai, Yota Nakai, Naoki Takami…·Toshihide Okajima

This paper describes the rational design of a cyclized TEV protease using SpyRing and AlphaFold, resulting in improved thermostability without loss of enzymatic activity.

2 citations

protein structureexperimental assay

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