Discover, rate, and verify biology preprints.

Sequential pathways preserve kinetochore integrity in meiosis

This study identifies three distinct pathways maintaining kinetochore integrity during meiosis in budding yeast, with distinct temporal requirements and interactions involving Ctf19cCCAN, Mif2CENP-C, and Cse4CENP-A-Okp1CENP-Q-Ame1CENP-U.

A Dynamic Stem Cell State Links Environmental Cues to Skeletal and Regenerative Fates

The paper identifies a dynamic stem cell state marked by Panx3 that responds to environmental cues and enables regeneration in aged and osteoarthritic joints.

ER stress-associated IRE1α signalling links impaired QPRT-dependent NAD⁺ biosynthesis to reduced oocyte competence

This study links ER stress and impaired NAD biosynthesis via QPRT to reduced oocyte quality and shows that NMN supplementation improves oocyte function and early embryonic development.

Proteome-wide profiling reveals compartment-specific RNA buffering of protein aggregation during stress

The paper uses R-DeeP to show stress-induced compartment-specific RNA buffering of protein aggregation in yeast, with nuclear RNA retention protecting nuclear proteins from aggregation.

Persistent and recyclable myosin II minifilaments drive cortical contractility in the early C. elegans embryo

This study uses advanced microscopy to show that myosin II minifilaments persist and recycle in C. elegans embryos, contributing to cortical contractility through stable, static interactions with actin.

A multipotent mouse distal airway secretory cell population that contributes to both airway and alveolar regeneration

This study identifies a multipotent secretory cell population in mouse distal airways that contributes to both airway and alveolar regeneration after injury.

Breaking the bottleneck: semi-automated quantification of actin filament dynamics

The paper introduces SMAUG, a semi-automated tool for analyzing actin filament dynamics from TIRF microscopy images, reducing analysis time while maintaining accuracy.

Multicellular Perturb-seq Reveals Lineage-Specific Transcriptional Control During Human Cardiac Organoid Development

This study uses Perturb-seq with CRISPRi to analyze how cardiac and vascular transcription factors coordinate lineage commitment and cross-lineage signaling in human cardiac organoids.

RhoGEF Solo regulates cell density-dependent keratin remodeling and desmosome maturation

This study shows that Solo regulates keratin-DSM interactions and DSM maturation in response to cell density through RhoGEF activity and actin remodeling.

Transition Fibers Play a Central Role in Import of a Glucose Transporter into the Flagellar Membrane

This study identifies KHARON as a component of transition fibers that facilitate the transport of the glucose transporter GT1 into the flagellar membrane via interaction with IFT trains.

Endothelial Cell Specific Thioredoxin 1-Dependent Signaling in Oxygen-1 Induced Retinopathy

This study shows that endothelial cell-specific TXN1 protects against oxygen-induced retinopathy by regulating proliferation and redox signaling.

Platycodin D2 Targets Vimentin to Suppress Fibroblast-Myofibroblast Transition: A Novel Therapeutic Strategy Against Pulmonary Fibrosis

This study identifies Platycodin D2 as a vimentin ligand that suppresses fibroblast-myofibroblast transition, offering a new therapeutic approach for pulmonary fibrosis.

Diversification of budding yeast polarization dynamics after compensatory experimental evolution

This study shows that evolution can restore cellular function without conserving microscopic organization, leading to novel molecular phenotypes in budding yeast.

Receptor-proximal phosphoform organization precedes β2 integrin activation

The paper quantifies phosphoform organization near β2 integrins and shows that signaling alters phosphosites while preserving spatial organization of proteins.

Altered Steroidogenesis in Response to Impaired Mitochondrial Protein Homeostasis

This study shows that mitochondrial proteostasis stress causes a biphasic cortisol response, with mild stress increasing and severe stress decreasing cortisol production, linking mitochondrial function to steroidogenesis in Parkinson's disease models.

Neural Activation of Human Primary Intestinal Enteroendocrine L-cells

This study identifies cholinergic and adrenergic neural signals that activate human intestinal L-cells, triggering hormone secretion and calcium waves.

XRN1 couples RNA granule remodelling to global mRNA decay triggered by the activity of a viral endoribonuclease

The paper investigates how XRN1 links RNA granule remodeling to global mRNA decay triggered by the viral endoribonuclease vhs, revealing a sequential model of translational arrest and degradation.

Systematic comparison of genetically encoded F-actin probes for visualizing actomyosin dynamics in vivo using C. elegans

The paper compares three F-actin probes in C. elegans, finding mStayGold offers best brightness and photostability, while mNeonGreen is suitable for photobleaching applications.

KDM6A is enzymatically active at the Golgi apparatus where it is necessary for maintenance of Golgi apparatus morphology

This study shows KDM6A is enzymatically active at the Golgi apparatus and is necessary for maintaining its morphology, independent of its nuclear function.

The Application of 3D Cryo-Volume Electron Microscopy to Visualize and Quantify the Photosynthetic Filamentous Cyanobacterium Anabaena

This paper uses cryo-vEM to visualize and quantify ultrastructural features of two Anabaena species without chemical pre-treatment.