Functional diversification of hybridoma produced antibodies by CRISPR/HDR genomic engineering
J. M. S. van der Schoot, F. L. Fennemann, M. Valente, Y. Dolen, I. M. Hagemans, A. M. D. Becker, C. M. Le Gall, D. van Dalen, A. Cevirgel, J. A. C. van Bruggen, M. Engelfriet, M. F. Fransen, T. Caval, A. E. H. Bentlage, M. Nederend, , F. A. Scheeren
This study presents a CRISPR/HDR platform to engineer hybridoma antibodies for diverse isotypes, formats, and species, enabling functional antibody diversification.
bioRxiv · Feb 15, 2019Bioengineering