Tuning the sensitivity of genetically encoded fluorescent potassium indicators through structure-guided and genome mining strategies
C. C. Torres Caban, M. Yang, C. Lai, L. Yang, F. Subach, B. O. Smith, K. D. Piatkevich, E. S. Boyden
The paper develops genetically encoded fluorescent potassium indicators with tunable binding affinities through structure-guided design and genome mining, showing varying Kd values in vitro and in cells.
bioRxiv · Oct 7, 2021Bioengineering