Nuclease dead Cas9 is a programmable roadblock for DNA replication
K. Whinn, G. Kaur, J. S. Lewis, G. Schauer, S. Müller, S. Jergic, H. Maynard, Z. Yan Gan, M. Naganbabu, M. P. Bruchez, M. O'Donnell, N. E. Dixon, A. M. van Oijen, H. Ghodke
The paper uses a modified Cas9 protein to create a programmable roadblock for studying DNA replication fork arrest in vitro and in vivo.
bioRxiv · Oct 29, 2018Biochemistry